Optimisation of ion trap parameters for the quantification of chlormequat by liquid chromatography/mass spectrometry and the application in the analysis of pear extracts

✍ Scribed by Catherine S Evans; James R Startin; David M Goodall; Brendan J Keely

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 141 KB
• Volume: 14
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(20000130)14:2<112::aid-rcm841>3.0.co;2-0

✦ Synopsis

Optimisation of the activation parameters for ion trap mass spectrometric analysis of the chlormequat cation using simplex optimisation enabled the product ion (m/z 58) response to be improved 1000-fold. A comparison of the sensitivity of the optimised ion trap mass spectrometer with that of a triple quadrupole mass spectrometer for liquid chromatography/tandem mass spectrometry (LC/MS/MS) showed that similar limits of detection (LODs) could be achieved. For the MS/MS transition of the 35 Cl precursor to the most abundant product, LODs were 0.8 ng cation mL À1 (0.004 mg cation kg À1 pear equivalent) and 1.0 ng cation mL À1 (0.005 mg cation kg À1 pear equivalent) on the triple quadrupole and ion trap instrument, respectively.