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Optimisation of collagen production in Pichia pastoris shake flask cultures by wireless monitoring and control

✍ Scribed by Maria Ruottinen; Martin Kögler; Monika Bollok; Antje Neubauer; Mirja Krause; Eija-Riitta Hämäläinen; Johanna Myllyharju; Antti Vasala; Peter Neubauer

Book ID: 118472109
Publisher: Elsevier Science
Year: 2007
Tongue: English
Weight: 49 KB
Volume: 131
Category: Article
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2007.07.122

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The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1 (AOX1) promoter. Accurate regulation of the methanol concentration in P. pastoris cultures is necessary to maintain induction, while preventi