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Optimisation of chemical protein cleavage for erythropoietin semi-synthesis using native chemical ligation

✍ Scribed by Richardson, Jonathan P.; Macmillan, Derek

Book ID
118740863
Publisher
Royal Society of Chemistry
Year
2008
Tongue
English
Weight
318 KB
Volume
6
Category
Article
ISSN
1477-0520

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## Abstract The process of native chemical ligation (NCL) is well described in the literature. An __N__‐terminal cysteine‐containing peptide reacts with a __C__‐terminal thioester‐containing peptide to yield a native amide bond after transesterification and acyl transfer. An __N__‐terminal cysteine