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Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples

โœ Scribed by Petra Vasickova; Petr Kralik; Iva Slana; Ivo Pavlik


Book ID
113768851
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
252 KB
Volume
180
Category
Article
ISSN
0166-0934

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## Abstract To compare the specificity and sensitivity of a realโ€time fluorescent RTโ€PCR assay with conventional RTโ€PCR, sera from 110 healthy blood donors, 120 patients with a clinical diagnosis of chronic hepatitis B, and 416 patients with nonโ€Aโ€C acute hepatitis, as well as serial dilutions of H