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Optimisation of a host/vector system for heterologous gene expression byHansenula polymorpha

✍ Scribed by Laurens N. Sierkstra; John M. A. Verbakel; C. Theo Verrips


Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
987 KB
Volume
19
Category
Article
ISSN
0172-8083

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A set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator. Expression from the promoter is regulated by tetracycline or derivatives. Various modalities of promoter and acti