## Abstract Since the amount of hepatitis C virus (HCV) RNA might be correlated with the degree of severity of hepatitis and response to treatment, quantitation of HCV RNA in serum was established using competitive polymerase chain reaction (PCR). Known amounts of a plasmid containing HCVβcDNA were
Optimisation and evaluation of a quantitative cherniluminescent polymerase chain reaction assay for hepatitis C virus RNA
β Scribed by K. Whitby; J.A. Garson
- Book ID
- 113256469
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 896 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0166-0934
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Although detection of hepatitis C virus RNA with polymerase chain reaction has become the standard for diagnosis, extensive application has been thwarted by polymerase chain reaction's labor intensiveness, risk of false-positive results through contamination and time required for individual assays.
We have developed a homogeneous quantitative assay of DNA/RNA by performing PCR in the presence of an oxazole yellow derivative, a fluorescent DNA intercalative dye, and monitoring the fluorescence intensity of the PCR reaction mixture during PCR cycles. We have demonstrated the applicability of thi