✦ LIBER ✦
Optimierung von Kulturbedingungen für Acinetobacter calcoaceticus beim Wachstum auf n-Alkanen in einem Laborfermentor
✍ Scribed by Dr. B. Fricke; R. Bergmann; H. Sorger; H. Aurich
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 467 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
The growth conditions for the cultivation of Acinetobacter calcoaceticus in a commercial laboratory fermenter have been optimized. This was done taking former results of shake cultures as a basis. At the end of the logarithmic phase up to 10 g/l dry weight were obtained.
The following culture conditions have been used: The medium contained 10 ml/l n‐alkane and 6 g/l NH~4~Cl. The pH was adjusted to 6.5 and pO~2~ employed to 70% saturation.
A 12 times higher amount of dry weight was observed compared with shake culture technique.