Escherichia coli and Salmonella typhimurium are closely related species of enteric bacteria, having diverged from 120 to 160 million years ago, according to the estimate of Ochman & Wilson (1987. J. Mol. Evol. 26, 74}86). In order to study base substitution mutations in the genomes of these bacteria
Optimality of codon usage in Escherichia coli due to load minimization
โ Scribed by Hamed Shateri Najafabadi; Hani Goodarzi; Noorossadat Torabi
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 201 KB
- Volume
- 237
- Category
- Article
- ISSN
- 0022-5193
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Possible translational pauses within the coat protein of the RNA bacteriophage MS2 were located on the basis of a distribution plot of rare codons and RNA secondary structure. It appeared that the position of certain codon pauses corresponds with the size of some nascent polypeptide intermediates, w
Evaluation of the yield of expression of exogeneous protein in transformed Escherichia coli cells by means of one-dimensional SDS-PAGE often leads to overestimation and miscalculation. For example, it is possible that proteins of similar size comigrate and thus mask the overexpressed product band. T