Optimal preparation of rhesus macaque blood for cytokine flow cytometric analysis
β Scribed by Laurel E. Nomura; Eileen D. deHaro; Louis N. Martin; Holden T. Maecker
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 265 KB
- Volume
- 53A
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Background: This study was undertaken in mice to develop a reproducible procedure of cell permeabilization, allowing intracellular protein staining by immunofluorescence (i.e., bcl-2) without losing surface labeling especially for lectins (i.e., b220 and peanut agglutinin [pna]). this article re
Enzyme inhibitors have been compared with conventional anticoagulants for the flow cytometric analysis of live leucocytes in whole blood. At 18 to 20Β°C in vitro, PPACK (60 pM), h h d i n (10 units ml-'), leupeptin (20 pg ml-l) and aprotinin (50 pg
A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solve
## Abstract ## Background Flow cytometric analysis of peripheral blood dendritic cells (PBDCs) and their myeloid (mDCs) and plasmacytoid (pDCs) subsets is a less invasive procedure that is acquiring growing clinical relevance. Because dendritic cells (DCs) lack unique lineage markers, current meth
## Background: Several sensitive surveillance tests reportedly have been used to detect occult neuroblastoma (nb) cells in peripheral blood (pb) and bone marrow (bm). they may be useful in monitoring minimal residual tumor cells. the authors report the feasibility and clinical usefulness of a sensi