Optimal oxygen tension for human lymphocytes in culture

✍ Scribed by V. Andersen; P. Hellung-Larsen; S. F. Sørensen

• Book ID: 102308368
• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 167 KB
• Volume: 72
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040720302

✦ Synopsis

Human lymphocytes stimulated with phytohaemagglutinin in vitro

show maximal I4C-thymidine incorporation when the gas phase of the cultures contains 20% 02. The rate of DNA synthesis is more affected by hyperoxic conditions than by hypoxia. Cell viability, defined by dye exclusion, is considerably less dependent on pO2 than is cellular replication.