✦ LIBER ✦
Optimal conditions for proliferation of bone marrow-derived mouse macrophages in culture: The roles of CSF-1, serum, Ca2+, and adherence
✍ Scribed by David A. Hume; Siamon Gordon
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 714 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
A method is described for the analysis of [3H]-thymidine incorporation in microtitre cultures of bone marrow-derived mouse macrophage responding to macrophage colony-stimulating factor (CSF-1). [3H]-thymidine incorporation depends on cell density, culture medium, and the concentration of CSF- 1 and serum, but is independent of Ca2+. Bone marrow-derived macrophages are strongly adherent, but adherence can be dissociated from [3H]- thymidine incorporation.