Optimal conditions for collection of blood samples for assay of α2-macroglobulin trypsin complex-like substance (MTLS)
✍ Scribed by Masanori Kato; Noriyuki Tatemichi; Shinobu Hayakawa; Satoru Naruse; Motoji Kitagawa; Yasuyuki Nakae; Tetsuo Hayakawa
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 343 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
We have developed an enzyme-linked immunosorbent assay (ELISA) for the specific quantification of a2-macroglobulintrypsin complex-like substance (MTLS). To exclude artifacts in measured values of MTLS, the conditions for collection of blood samples are critical. In the present study, we have determined the optimal conditions for blood collection and investigated the role of MTLS as a clinical tool for diagnosis in pancreatitis.
Results obtained are as follows: (1) MTLS levels of all sera were more than 10-fold higher than the corresponding plasma; (2) MTLS levels of heparinized plasma were the lowest among plasma with three anticoagulants (sodium citrate, sodium EDTAand heparin); (3) some kinds of blood collection tubes containing heparin were not suitable for the sampling; (4) MTLS values of plasma ob-tained by blood collection tubes containing TrasyIoKB and sodium EDTA were demonstrated more stable and lower than those obtained by heparin tubes; and (5) under these conditions, we can exclude elevation of MTLS values caused by inappropriate blood sampling and find the time course of the elevation reflecting clinical course of a patient with acute pancreatitis and a patient after endoscopic retrograde cholangiopancreatography (ERCP).
The optimal conditions for collection of blood samples were as follows. Blood sampling should be performed by blood collection tubes containing Trasylol@ (50 pl/ml blood) and sodium EDTA (1.5 mg/ml blood). The samples were immediately stored at 4°C and centrifuged at 3,000 rpm for 15 min. The plasma were stored in plastic tubes at 4°C until assayed.