Optical sensing of pH and pCO2 using phase-modulation fluorimetry and resonance energy transfer

✍ Scribed by Joseph R. Lakowicz; Henryk Szmacinski; Mutlu Karakelle

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 645 KB
• Volume: 272
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80567-5

✦ Synopsis

We have developed opt& sensors for pH and/or pC0, based on phase shift and modulation measurements of time-resolved fluorescence energy transfer pH-msenatrve donors and pH-sensltlve acceptors were encased m C&-permeable silicon membranes The extent of energy transfer depends on the pH-dependent absorption spectra of the acceptor The measurements were made msensdlve to inner-filter effects of the total intensity by using the phase-modulation method to determme the donor decay times The general apphcaluhty of this method was demonstrated using three donor-acceptor pairs, all of which can be excited urlth an mexpenswe green 543-nm He-Ne laser