Optical rotatory dispersion of egg proteins. I. Ovalbumin, conalbumin, ovomucoid, and lysozyme

✍ Scribed by Yoshio Tomimatsu; William Gaffield

• Book ID: 102760152
• Publisher: Wiley (John Wiley & Sons)
• Year: 1965
• Tongue: English
• Weight: 520 KB
• Volume: 3
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1965.360030502

✦ Synopsis

Optical rotatory dispersions (ORD) of ovalbumin, conalbumin, ovomucoid, and lysozyme (muramidase) were measured in aqueous solution over a wavelength range of 195-600 mp. The dispersion curves obeyed a one-term Drude equation above 300 mk The strong Cotton effects in the ultraviolet showed peaks and troughs at about the same wavelength for all four proteins, but the crossover point for the 225 mp Cotton effect for ovomucoid was appreciably lower than for the other proteins. Helical contents calculated by using the Moffitt equation, the trough value of the 225 ma Cotton effect, and the expressions for helical content recently proposed by Schechter and Blout ranged from 5 to 34%. Except for ovomucoid, the trough value of the 225 mp Cotton effect. gave the lowest helical content.