✦ LIBER ✦

Optical rotation detection for reversed phase HPLC: Investigation of solvent effects

✍ Scribed by Jonathan L. Sims; Judith A. Carreira; Ryan A. Bragg

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 145 KB
Volume: 9
Category: Article
ISSN: 0899-0042
DOI: 10.1002/(sici)1520-636x(1997)9:2<122::aid-chir8>3.0.co;2-j

No coin nor oath required. For personal study only.

✦ Synopsis

Investigations into the parameters affecting the sensitivity of polarimetric detectors for the determination of enantiomeric excess by achiral HPLC are presented. The results obtained showed that the specific optical rotation of an analyte was highly sensitive to the methanol content of the mobile phase. The purpose of this short communication is to demonstrate that ''off-line'' optical rotation measurements are a necessary part of the method development process for successful use of polarimetric detectors. Chirality 9: 122-125, 1997.

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