Optical Imaging Techniques in Cell Biology

✍ Scribed by Cox, Guy

• Publisher: CRC Press
• Year: 2012
• Tongue: English
• Leaves: 305
• Edition: 2
• Category: Library

✦ Synopsis

Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.

✦ Table of Contents

Front Cover
Contents
Introduction: The Optical Microscope in Cell Biology
Contributors
Acknowledgments
Chapter 1 - The Light Microscope
Chapter 2 - Optical Contrasting Techniques
Chapter 3 - Fluorescence and Fluorescence Microscopy
Chapter 4 - Image Capture
Chapter 5 - The Confocal Microscope
Chapter 6 - The Digital Image
Chapter 7 - Aberrations and Their Consequences
Chapter 8 - Nonlinear Microscopy
Chapter 9 - High-Speed Confocal Microscopy
Chapter 10 - Deconvolution and Image Processing
Chapter 11 - Three-Dimensional Imaging: Stereoscopy and Reconstruction
Chapter 12 - Green Fluorescent Protein
Chapter 13 - Fluorescent Staining
Chapter 14 - Quantitative Fluorescence
Chapter 15 - Advanced Fluorescence Techniques: FLIM, FRET, and FCS
Chapter 16 - Evanescent Wave Microscopy
Chapter 17 - Beyond the Diffraction Limit
Appendix A: Microscope Care and Maintenance
Appendix B: Keeping Cells Alive under the Microscope
Appendix C: Antibody Labeling of Plant and Animal Cells: Tips and Sample Schedules
Appendix D: Image Processing with ImageJ
Back Cover

✦ Subjects

Cytological Techniques methods Cytology Imaging systems in biology Microscopy