𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Opsonization of polyethylene wear particles regulates macrophage and osteoblast responsesin vitro

✍ Scribed by Elfick, A.P.D. ;Green, S.M. ;McCaskie, A.W. ;Birch, M.A.

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
120 KB
Volume
71B
Category
Article
ISSN
0021-9304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The cellular reaction to wear debris may result in the failure of an artificial joint's fixation to the skeleton. The influence of debris opsinization on cell activity has received little attention. This study seeks to establish whether different proteinaceous culture environments may invoke variant cellular responses to debris challenge. Consideration of the zeta potential of a low density polyethylene particle group and an ex vitro ultrahigh molecular weight polyethylene particle group revealed that the nature of the protein adsorbants is related to the concentration of the proteins in solution. Furthermore, the composition of the adsorbed layer was shown to vary with the spectra of proteins in solution. In standard cell culture conditions zeta potential approached zero, indicating the high probability of particle agglomeration. Cell challenge studies with U937 macrophages showed that BSA and FCS protein adsorption mediated increased cell adhesion, while bovine IgG showed little change over control values. No changes in behavior of osteoblastic cells were observed in similar experiments. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 71B: 244–251, 2004

📜 SIMILAR VOLUMES

Human serum opsonization of orthopedic b
Human serum opsonization of orthopedic biomaterial particles: Protein-binding and monocyte/macrophage activationin vitro
✍ Sun, Doo-Hoon ;Trindade, Michael C. D. ;Nakashima, Yasuharu ;Maloney, William J. 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 255 KB

## Abstract Wear particles generated after total joint arthroplasty activate monocyte/macrophages and incite formation of a granulomatous periprosthetic tissue associated with bone loss and implant loosening. This study tested the hypothesis that selective opsonization of orthopedic implant biomate

In vitro assessment of osteoblast and ma
In vitro assessment of osteoblast and macrophage mobility in presence of β-TCP particles by videomicroscopy
✍ Johanne Beuvelot; Florence Pascaretti-Grizon; Robert Filmon; Marie-Fraçoise More 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 648 KB

## Abstract β‐TCP is widely used to repair bone defects due to its good biocompatibility, macroporosity (favoring bone ingrowth) and bioresorbability. However, cell interactions with the biomaterial at the first times of implantation remain largely unknown. We have observed cell behaviors in direct

Binding of proteins to ultra high molecu
Binding of proteins to ultra high molecular weight polyethylene wear particles as a possible mechanism of macrophage and lymphocyte activation
✍ E. Zolotarevová; J. Hudeček; M. Špundová; G. Entlicher 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 207 KB

## Abstract Binding of five human plasma proteins (IgG, serum albumin, α~1~–acid glycoprotein, holo‐transferrin, α~1~–antitrypsin) to ultra high molecular weight polyethylene wear particles (0.1–10 μm) isolated from hip periprosthetic tissues was studied __in vitro__. All tested plasma proteins wer

Morphology ofIn Vitro generated ultrahig
Morphology ofIn Vitro generated ultrahigh molecular weight polyethylene wear particles as a function of contact conditions and material parameters
✍ Landry, Michael E. ;Blanchard, Cheryl R. ;Mabrey, Jay D. ;Wang, Xiaodu ;Agrawal, 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 266 KB 👁 2 views

Osteolysis in total joint replacements has been linked to ultrahigh molecular weight polyethylene (UHMWPE) wear particles. Although the biological response to these UHMWPE particles is poorly understood at present, it has been demonstrated that particle size and morphology are important factors in s