Opposing mitogenic regulation by PACAP in sympathetic and cerebral cortical precursors correlates with differential expression of PACAP receptor (PAC1-R) isoforms
✍ Scribed by Nairu Lu; Renping Zhou; Emanuel DiCicco-Bloom
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 180 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0360-4012
No coin nor oath required. For personal study only.
✦ Synopsis
pathway. In contrast, cerebral cortical precursors express primarily the ''short'' (non-insert) receptor isoform and exhibit increased cAMP levels alone following PACAP treatment. Thus, opposing mitogenic regulation in sympathetic and cortical precursors correlates with differential receptor isoform expression and distinct second-messenger signaling. In addition to receptor, PACAP ligand mRNA was expressed by both populations, suggesting that the peptide is produced and acts locally to regulate precursor proliferation. These observations indicate that the PACAP ligand/receptor system is expressed in both the peripheral and central nervous system during development. More generally, these studies suggest that widely expressed extracellular factors mediate region-specific neurogenesis by activating lineagerestricted receptor isoforms and intracellular pathways.