Opportunities for ultra-high resolution analysis of essential oils using comprehensive two-dimensional gas chromatography: a review

✍ Scribed by Robert Shellie; Philip Marriott

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 624 KB
• Volume: 18
• Category: Article
• ISSN: 0882-5734
• DOI: 10.1002/ffj.1225

✦ Synopsis

Abstract

In comprehensive 2D gas chromatography, the entire sample is simultaneously subjected to analysis on two capillary columns. By using a suitable modulation interface between the primary and secondary columns, hundreds of fast, second‐dimension chromatograms are produced. The data from these chromatograms are treated such that a 3D surface plot or a 2D contour plot of the components' individual retention times, on each column, as well as peak responses, are represented. In a properly tuned comprehensive 2D chromatogram, the individual sample components are spread throughout a 2D separation space, providing a significant increase in the probability of resolving a greater number of sample components without increasing the analysis time. Comprehensive 2D–GC has proved useful for high‐resolution conventional essential oil analysis as well as high‐resolution enantioselective essential oil analysis. Combining comprehensive 2D–GC with either a quadrupole or time‐of‐flight mass spectrometer gives a powerful 3D analysis technique, which is extremely effective for complex sample analysis. The present status and opportunities arising from these ultra‐high resolution approaches are discussed herein. Copyright © 2003 John Wiley & Sons, Ltd.