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Opioid peptide receptor studies. 14. Stereochemistry determines agonist efficacy and intrinsic efficacy in the [35S]GTP-γ-S functional binding assay

✍ Scribed by Heng Xu; Akihiro Hashimoto; Kenner C. Rice; Arthur E. Jacobson; James B. Thomas; F. Ivy Carroll; Josephine Lai; Richard B. Rothman


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
99 KB
Volume
39
Category
Article
ISSN
0887-4476

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✍ Dana V. Romero; John S. Partilla; Qaio-Xi Zheng; Simone O. Heyliger; Qing Ni; Ke 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 511 KB

We utilized the [ 35 S]-GTP-␥-S functional binding assay to determine the selectivity of opioid receptor agonists in guinea pig caudate membranes. The study focused on two opioid agonists used for treating opioid-dependent patients: methadone and buprenorphine. Selective antagonists were used to gen