Operational variables of size exclusion columns with semirigid and rigid packings
β Scribed by Mukherji, Anil K.
- Book ID
- 102345603
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 243 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Abstract
Systematic studies on operation variables affecting GPC separations using the semirigid Microstyragel (Waters Associates) and the rigid silica packings (DuPont) of about equivalent porosity are reported. The dependence of (a) flow rate, (b) injection volume, and (c) concentration on elution volume and theoretical plates are examined.
π SIMILAR VOLUMES
## Abstract Columns with inner diameters of 50 and 28 ΞΌm were slurryβpacked with 4β5 ΞΌm sizeβexclusion chromatography particles (Du Pont GF 250 Zorbax Bioseries) and compared with a commercially prepared 9.4βmm i.d. column that was packed with the same particles. The columns were compared on the ba
## Abstract Relationships have been derived between the spreading factor and the respective slopes of actual and effective linear calibrations in sizeβexclusion chromatography. On this basis a new calibration procedure has been developed for determining simultaneously the dependences of sample mole