Operational modes for transient isotachophoretic preconcentration-capillary zone electrophoresis and detectable concentration of rare earth ions

✍ Scribed by Takeshi Hirokawa; Hikaru Okamoto; Natsuki Ikuta

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 142 KB
• Volume: 22
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/1522-2683(200109)22:16<3483::aid-elps3483>3.0.co;2-o

✦ Synopsis

Operational modes for transient isotachophoretic preconcentration-capillary zone electrophoresis and detectable concentration of rare earth ions

Operational modes for transient isotachophoretic preconcentration capillary zone electrophoresis (tr-ITP-CZE) were studied by using 5 mM and 0.5 mM rare earth mixtures as analytes in comparison with field-enhanced sample stacking. After examination of several operational modes for tr-ITP, it was found that tr-ITP effectively occured even if both the leading electrolyte and the terminating electrolyte were injected after the sample plug. This was explained as the result of a field-enhanced stacking for both sample components and the leading and terminating ions. The observed theoretical plate numbers were 4-20 times higher than those obtained by normal stacking; and the estimated low limit of detectable concentration of rare-earth elements (REE) was ca. 0.1 mM which was 2.5 times lower compared to normal stacking. For the 0.5 mM sample, a concentration factor of 20 000 could be achieved after only tr-ITP.