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Operating microscope use in scleral buckling Correspondence

✍ Scribed by Singh, A; Stewart, J M


Book ID
110036970
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
43 KB
Volume
21
Category
Article
ISSN
0950-222X

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## Abstract We describe a method to obtain the brightness and number of molecules at each pixel of an image stack obtained with a laser scanning microscope. The method is based on intensity fluctuations due to the diffusion of molecules in a pixel. For a detector operating in the analog mode, the v