Oocyte-surface factor responsible for 1-methyladenine-lnduced oocyte maturation in starfish

✍ Scribed by Morisawa, Masaaki ;Kanatani, Haruo

• Book ID: 102529042
• Publisher: Wiley (John Wiley & Sons)
• Year: 1978
• Weight: 417 KB
• Volume: 1
• Category: Article
• ISSN: 0148-7280
• DOI: 10.1002/mrd.1120010209

✦ Synopsis

Abstract

Fully grown oocytes of the starfish Asterina pectinifera, undergo breakdown of their germinal vesicles and subsequent maturation on treatment with 1‐methyladenine (1‐MeAde). However, oocytes treated with seawater containing 0.010% Triton X‐100 lost the capacity to respond to 1‐MeAde and their germinal vesicles remained intact. These decapacitated oocytes once again ac‐quired the capacity to respond to l‐Me Ade when they were incubated in sea water containing the extract of fully grown oocytes treated with Triton X‐100, from which the Triton X‐100 was removed after extraction by means of Bio‐Beads SM‐2 (TXE). Recovery of the capacity was also observed after washing such TXE‐treated oocytes with sea water. These results suggest that some factor (probably 1‐MeAde receptor or its fragment), extracted from the oocyte surface (plasma mem‐brane) by nonionic detergent, was reconstituted on the oocyte surface so that the capacity of the oocytes to respond to 1‐MeAde was recovered. The factor was heat‐stable and resistant to treat‐ment with proteolytic enzymes.