Online Pre-Column Derivatization with Chromatographic Separation to Determine Folic Acid
β Scribed by Emara, S.; Masujima, T.; Zarad, W.; Kamal, M.; EI-Bagary, R.
- Book ID
- 118061786
- Publisher
- Preston Publications
- Year
- 2012
- Tongue
- English
- Weight
- 268 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0021-9665
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π SIMILAR VOLUMES
A selective and sensitive high-performance liquid chromatographic method is described for determination of hydralazine and its metabolites in human plasma. The method involves pre-column derivatization with 2-hydroxy-1-naphthaldehyde at pH 1.2. The reaction product and Methyl Red used as internal st
adducts are destroyed coelutes with the isooctane. Peaks of ## Short Communications Table 1 retention time 2.63,5.44, and 7.65 minutes are from the reagent. Even though these peaksarequitelarge,theydo not interferewith the analysis of the esters. ## Retention data for tert-butyldimethylsilyl est