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Online detection of feed demand in high cell density cultures of Escherichia coli by measurement of changes in dissolved oxygen transients in complex media

✍ Scribed by Victoria S. Whiffin; Michael J. Cooney; Ralf Cord-Ruwisch


Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
484 KB
Volume
85
Category
Article
ISSN
0006-3592

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On-line detection of acetate formation i
✍ Mats Γ…kesson; Eva Nordberg Karlsson; Per Hagander; Jan Peter Axelsson; Anita Toc πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 183 KB πŸ‘ 2 views

Recombinant protein production in Escherichia coli can be significantly reduced by acetate accumula- tion. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate format