On-line detection of acetate formation i
β
Mats Γ
kesson; Eva Nordberg Karlsson; Per Hagander; Jan Peter Axelsson; Anita Toc
π
Article
π
1999
π
John Wiley and Sons
π
English
β 183 KB
π 2 views
Recombinant protein production in Escherichia coli can be significantly reduced by acetate accumula- tion. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate format