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Online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization

✍ Scribed by Maggie P. Y. Lam; S. O. Siu; Edward Lau; Xiuli Mao; H. Z. Sun; Philip C. N. Chiu; William S. B. Yeung; David M. Cox; Ivan K. Chu


Publisher
Springer
Year
2010
Tongue
English
Weight
794 KB
Volume
398
Category
Article
ISSN
1618-2650

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## Abstract A new technique for coupling reversed‐phase liquid chromatography (RPLC) with gas chromatography is described. A fraction eluting from an RPLC column is trapped on a short column packed with polymeric adsorbent. After the mobile phase has been displaced with water, the analytes are deso