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Online CIEF-ESI-MS in glycerol–water media with a view to hydrophobic protein applications

✍ Scribed by Meriem Mokaddem; Pierre Gareil; Anne Varenne


Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
332 KB
Volume
30
Category
Article
ISSN
0173-0835

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## Abstract Capillary isoelectric focusing (CIEF) separations are usually performed with neutral coated fused‐silica capillaries in aqueous anticonvective media. Glycerol, a very viscous solvent (η = 945 mPa·s at 25°C), known to help stabilize any kind of proteins and solubilize hydrophobic ones, w