𝔖 Bobbio Scriptorium
✦   LIBER   ✦

One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

✍ Scribed by Jeong, Jae Yeon (author);Yim, Hyung Soon (author);Ryu, Ji Young (author);Lee, Hyun Sook (author);Lee, Jung Hyun (author);Seen, Dong Seung (author);Kang, Sung Gyun (author)


Book ID
111945626
Publisher
American Society for Microbiology
Year
2012
Tongue
English
Weight
822 KB
Volume
78
Category
Article
ISSN
0099-2240

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


A Method for the Large-Scale Cloning of
✍ Bruno Pichon; Dany Mercan; ValΓ©rie Pouillon; Christiane Christophe-Hobertus; Dan πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 169 KB

We describe here a selection strategy allowing the cloning of sequences that contain a functional nuclear targeting signal. Our method relies on the use of green fluorescent protein fusion proteins to identify nuclear targeting sequences. Transfected cells expressing nuclear protein fusions were iso