One-step isolation of γ-oryzanol from rice bran oil by non-aqueous hydrostatic countercurrent chromatography
✍ Scribed by Apostolis Angelis; Aurélie Urbain; Maria Halabalaki; Nektarios Aligiannis; Alexios-Leandros Skaltsounis
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 364 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
The value‐added γ‐oryzanol was purified in one step from crude rice bran oil (RBO) using a preparative hydrostatic countercurrent chromatography (hydrostatic CCC) method, operating in the dual mode. The fractionation was performed using a non‐aqueous biphasic solvent system consisting of heptane–acetonitrile–butanol (1.8:1.4:0.7, v/v/v), leading rapidly to the target compounds. Transfer of the analytical CCC method to large‐scale isolation was also carried out yielding a high quantity‐high purity fraction of γ‐oryzanol. In addition, a fraction of hydroxylated triterpene alcohol ferulates (polar γ‐oryzanol) was clearly separated and obtained. Furthermore, a fast HPLC‐APCI(±)‐HRMS method was developed and applied for the identification of γ‐oryzanol as well as the polar γ‐oryzanol in RBO and the resulting fractions. The purity of γ‐oryzanol fraction was estimated as 97% based on HPLC‐APCI‐HRMS analysis.