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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

โœ Scribed by Datsenko, K. A.

Book ID
111923923
Publisher
National Academy of Sciences
Year
2000
Tongue
English
Weight
509 KB
Volume
97
Category
Article
ISSN
0027-8424

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Chromosomal location of gene governing t
Chromosomal location of gene governing the trehalose utilization in Escherichia coli K12
โœ Becerra de Lares, L. ;Ratouchniak, J. ;Casse, F. ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 308 KB

Several mutants unable to utilize trehalose were isolated from Escherichia coli, Their genetic analysis led to determine the following gene order on the chromosomal map: pur B-dad R-tre-hem a-trp. Furthermore, the tre gene belongs to the inversion of the trp chromosomal region between E. coli and S.