One-Step Cationic Poly(D,L-lactic acid) Particle Synthesis by Diafiltration and Interactions with Plasmid DNA

✍ Scribed by Isabelle Messai; Thierry Delair

• Book ID: 102491626
• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 227 KB
• Volume: 290
• Category: Article
• ISSN: 1438-7492
• DOI: 10.1002/mame.200500129

✦ Synopsis

Abstract

Summary: Diafiltration was used for the one step preparation of cationic poly(D,L‐lactic acid) particles in the presence of poly(ethylenimine) (PEI). First, parameters impacting the formation of plain PLA particles were investigated: the chemical nature of the solvent and the polymer concentration within the organic phase. Then, PEI was added to the organic solution and this mixture was subsequently dialyzed against water. The molecular architecture of the polycation was essential and colloidal particles were only obtained with a branched PEI of 25 000 g · mol^−1^. Increasing the PEI concentration in the mixture led to a particle mean size reduction and a zeta‐potential increase. In any case, a maximum of 8% of the initial PEI input was recovered at the interface. Finally, the study of the interactions of these colloids with DNA showed that the amount of DNA bound at the saturation plateau increased with increasing inputs of PEI in the formulation.

SEM micrograph of cationic poly(D,L‐lactic acid) particles.

magnified imageSEM micrograph of cationic poly(D,L‐lactic acid) particles.