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One-step amino acid selective isotope labeling of proteins in prototrophic Escherichia coli strains

✍ Scribed by Christopher O’Grady; Benjamin L. Rempel; Akosiererem Sokaribo; Sergiy Nokhrin; Oleg Y. Dmitriev


Book ID
113412484
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
372 KB
Volume
426
Category
Article
ISSN
0003-2697

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An isogenic pair of Escherichia coli mutants (relA+ tufB valSts and relA1 tufB valSts) has been cultured at several temperatures to establish various degrees of limitation for valyl-tRNA synthetase. The biosynthetic rate of 16 identifiable proteins, most of which are components of the transcription