One-pot enzymatic synthesis of docosahexaenoic acid-rich triacylglycerols at the sn-1(3) position using by-product from selective hydrolysis of tuna oil
✍ Scribed by Toshihiro Nagao; Yomi Watanabe; Kazuaki Maruyama; Yuusuke Momokawa; Noriaki Kishimoto; Yuji Shimada
- Publisher
- Elsevier
- Year
- 2011
- Tongue
- English
- Weight
- 479 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1871-6784
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✦ Synopsis
Docosahexaenoic acid (DHA)-rich oil has been industrially produced by selective hydrolysis of tuna oil with a lipase that acts weakly on DHA. The free fatty acids (FFAs) generated in this process as by-products contain a high DHA concentration (46wt%) but are treated as industrial waste. This study attempted to reuse these by-product FFAs using a one-pot process, and succeeded in producing triacylglycerols (TAGs) through the esterification of the by-product FFAs with glycerol using immobilized Rhizomucor miehei lipase. Regiospecific analysis of the resulting TAGs showed that the content of DHA at the sn-1(3) position (51.7mol%) was higher than the content of DHA at the sn-2 position (17.3mol%). The DHA distribution in TAGs synthesized in this study was similar to the DHA distribution in TAGs from seal oil.