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One- and two-dimensional nmr spectral analysis of the consequences of single amino acid replacements in proteins

✍ Scribed by John L. Markley; David H. Croll; R. Krishnamoorthi; Gilberto Ortiz-Polo; William M. Westler; W. C. Bogard Jr.; M. Laskowski Jr.


Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
996 KB
Volume
30
Category
Article
ISSN
0730-2312

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✦ Synopsis


Abbreviations used: OMXXX3, unglycosylated ovomucoid third domain isolated following limited proteolysis of native ovomucoid by staphylococcal proteinase (unless noted otherwise) from the following species of birds: OMTKY3, turkey (Meleagris gallopavo); OMIPF3, [Indian] peafowl (Pavo crisfatus); OMSVP3, silver pheasant (Lophura nycthemera); OMGMQ3, Gambel's quail (Lophortyx gambelii); OMJPQ3, Japanese quail (Corurnix cotumix japonica); OMCHIS, chicken (Gallus gallus); OMFTD3, fulvous tree duck (Dendrocygna bicolor). COSY, homonuclear two-dimensional correlated spectroscopy; NOESY, two-dimensional nuclear Overhauser effect spectroscopy. pH*, uncorrected glass electrode pH meter reading of a sample solution containing *H20 made following calibration of the instrument with standard 'H20 buffers; 2DFT, two-dimensional Fourier transform; FID, free induction decay; TSP, 3-(trimethylsilyl)propionic acid-2, 2,3,3-2H (sodium salt); DSS, 3-(trimethylsilyl)-1-propanesulfonic acid (sodium salt); TMS, tetramethylsilane.


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## Abstract Cobalt(III) tetramethylchiroporphyrin, CoCl(TMCP), is a useful chiral shift reagent for structure attribution, absolute configuration assignment and enantiomeric excess determination of amino acid methyl esters by ^1^H NMR spectroscopy. However, it has two axial sites available for amin