Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures
✍ Scribed by Tomo Ehashi; Hirotoshi Miyoshi; Norio Ohshima
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 267 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
In order to develop a tissue engineered bioartificial liver (BAL), long‐term three‐dimensional (3‐D) culture of fetal liver cells (FLCs) utilizing porous polymer as a scaffold was performed for up to 1 month. The effects of the basal medium and supplementation with oncostatin M (OSM) on the proliferation and differentiation of mouse FLCs were examined in both 3‐D culture and conventional monolayer dish culture. Compared with monolayer culture, cell numbers and hepatic function of FLCs were better maintained by 3‐D culture. When two kinds of basal media were tested in this study, Williams' medium E (WE) was superior to minimum essential medium alpha (αMEM) in expressing hepatic function of FLCs in both 3‐D and monolayer cultures, although higher cell densities were obtained with αMEM. OSM potently stimulated both cell growth and metabolic activity, especially in 3‐D culture. When WE supplemented with OSM was used for 3‐D culture, albumin secretion by FLCs increased dramatically after day 5, and a high level of secretion was maintained until the end of culture. During a period of over 1 month, no decrease of albumin secretion was observed. In conclusion, this 3‐D culture method was expected to be one of the realistic attempts to develop a tissue engineered BAL. © 2004 Wiley‐Liss, Inc.