On.column pre-concentration of alcohol dehydrogenase in capillary electrophoresis

✍ Scribed by Shee-Ann Leung; Andrew J. de Mello

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 116 KB
• Volume: 25
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200290006

✦ Synopsis

Abstract

The analysis of alcohol dehydrogenase (ADH) at low concentration using capillary electrophoresis is described. Several simple and effective ways to improve detection limits and sensitivity are investigated. These include large volume sample stacking, head column field amplified sample stacking, and sweeping. Results indicate that by using a combination of head‐column field amplified sample stacking and sweeping, fluorescently labelled alcohol dehydrogenase can be pre‐concentrated online by dissolving samples in water or other low conductivity matrices, and injecting into a high conductivity micellar buffer. The abrupt changes in conductivity cause narrowing of the analyte length and thus enhance the detection sensitivity. Combination of this approach with laser induced fluorescence detection yields a limit of detection of 5×10^–13^ M. Both qualitative and quantitative aspects of this method are investigated.