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On the Use of Fluorescent Probes to Estimate Free Mg2+in the Matrix of Heart Mitochondria

✍ Scribed by Dennis W. Jung; Clifford J. Chapman; Kemal Baysal; Douglas R. Pfeiffer; Gerald P. Brierley

Book ID
115566142
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
262 KB
Volume
332
Category
Article
ISSN
0003-9861

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Fura-2 has been used to measure intracellular Ca2+ with great success in a variety of cell and subcellular preparations, including synaptosomes. There is, however, a great deal of variability in the reported estimates of resting intrasynaptosomal Ca2+ ([Ca2 + li). Fura-2 AM is highly lipophilic and