On the site specific recombination of phage 16-3 of Rhizobium meliloti: identification of genetic elements and att recombinations

✍ Scribed by Olasz, Ferene ;Dorgai, László ;Papp, Péter ;Hermesz, Edit ;Kósa, Edit ;Orosz, László

• Publisher: Springer
• Year: 1985
• Tongue: English
• Weight: 990 KB
• Volume: 201
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00425673

✦ Synopsis

The genome segment carrying the activities int and xis, responsible for integration and excision of phage 16-3, have been identified and cloned. Mutants were isolated, permitting the investigation of int, xis and att sites (attP, attR, attB) in trans arrangements. The efficiency and role of intand xis-promoted reactions and of homologous recombination in the formation of lysogenic cells are established. The possible use of the cloned int-attP chromosomal segment in the manipulation of Rhizobium meliloti is discussed.