## Abstract There is recent interest in recovery of periodontopathogenic bacteria from arterial and bronchial tissues to identify a link between periodontal and cardiovascular or pulmonary diseases. This interest could provide a useful clinical correlation exercise for gross anatomy. Our objective
On the recovery of bacteria from freezing
β Scribed by Jan Arpai
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 578 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0233-111X
No coin nor oath required. For personal study only.
β¦ Synopsis
On the recovery of bacteria from freezing JAN ARPAI (Eingegangen am 5. 7. 63
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## Abstract **BACKGROUND:** Disaccharides are, in general, the first choice as formulation compounds when freezeβdrying microorganisms. Although polysaccharides and other biopolymers are considered too large to stabilise and interact with cell components in the same beneficial way as disaccharides,
A procedure for quick and simple elution of DNA from agarose gels is presented. After electrophoresis, bands of interest are cut out of the gel and the slices are equilibrated in a neutral salt buffer. The slices are then frozen and centrifuged through a filtration assembly whereby the DNA-containin