✦ LIBER ✦

On the properties and utility of a membrane filter assay in the study of isoleucyl-tRNA synthetase

✍ Scribed by Michael Yarus; Paul Berg

Publisher: Elsevier Science
Year: 1970
Tongue: English
Weight: 858 KB
Volume: 35
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(70)90207-1

No coin nor oath required. For personal study only.

✦ Synopsis

There are many biochemical systems currently under study whose action consists of, or depends on, the ability of proteins to specifically bind another molecule. The availability of a method to measure binding quickly and quantitatively is an important aid in studying these systems. In many cases, the properties of nitrocellulose filters may be exploited to provide such an assay. These properties have already been used to some extent (e.g., l-4), but could be useful in other systems also. In this paper, we hope to aid the development of other assays by showing the effect of environmental variables on such a situation, the binding

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