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On the modification of sulfhydryl groups by 4-cyclopentene-1,3-dione

✍ Scribed by E. Toro-Goyco; C. Zuazaga de Ortiz; J. del Castillo


Book ID
112653026
Publisher
Springer
Year
1978
Tongue
English
Weight
231 KB
Volume
34
Category
Article
ISSN
1420-682X

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Mapping and analysis of protein sulfhydr
✍ Joseph Andrew Gardner; Kathleen Shive Matthews πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 401 KB

A method for identifying cysteine-containing peptides in proteins is presented using 2-bromoacetamido-4-nitrophenol (BNP) to introduce an easily detectable probe. The formation of a covalent bond between the protein sulfhydryl group and the acetamido moiety of BNP introduces a chromophore with an ab