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On the modeling of arginine-bound carboxylates: A case study with Pyruvate Formate-Lyase

✍ Scribed by Karmen Čondić-Jurkić; V. Tamara Perchyonok; Hendrik Zipse; David M. Smith


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
369 KB
Volume
29
Category
Article
ISSN
0192-8651

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✦ Synopsis


Abstract

High level ab initio and density functional calculations have been employed to determine the most appropriate manner in which to truncate an arginine‐bound carboxylate motif, using the substrate mechanism of Pyruvate Formate‐Lyase as a case study. The results show that, both qualitatively and quantitatively, a neutral carboxylic acid provides a more realistic approximation to the salt bridge arrangement than does a bare anionic carboxylate substituent. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2008