On the Mechanism of Catalysis by a Metal-Free Hydrogenase from Methanogenic Archaea: Enzymatic Transformation of H2 without a Metal and Its Analogy to the Chemistry of Alkanes in Superacidic Solution
✍ Scribed by Prof. Dr. Albrecht Berkessel; Prof. Dr. Rudolf K. Thauer
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 506 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0044-8249
No coin nor oath required. For personal study only.
✦ Synopsis
I hc kincllc inc~iwi-eiiieiif\ &&ere performed under pseudo-first-oi-dcr conditions a t I M ioiiic ~Ireiiflli (NaNO,). Solutions of the ligand end the coniplexes were hullcred ~i t l i pho\phatl: at pH =7. The rate of the reaction is independent of the pH 111 thi\ irangc ( I ] The p 'iiluc?, for the diffcrent complexes are 9.78 i R = CH,). t i I Y t 1 r \ut of lpr<>praiii\ [I?]. 0.06 (I( = ('lI:C'l), and x. IR = CF,CH2). conscqurntly a possible change in pH due to the clfi.ct of pressure on the buffer \ill not affect the iiie.asurud d.itn o r thc inechanism of the reaction [ 3 ] . The initial complex conceiitriition w a s ( 2 4 ) x 1 i l ~2 c i . and the initial ligind cwxentratioii v x i e d hclwccn 5 x 1W' and 10 x 1 K ' hi. The reactioiis u'ere Iblloned by the change in .ihwrhancc in the visible spectrum hetwecn 340 and 380 nm [I .~ 31 Received Ma). 11. l Y Y 5 [.?7Y741E]