On the kinetics of dextransucrase and dextran synthesis in batch reactors
β Scribed by J. P. Martinez-Espindola; C. A. Lopez-Munguia
- Book ID
- 105114873
- Publisher
- Springer Netherlands
- Year
- 1985
- Tongue
- English
- Weight
- 196 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0141-5492
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## Abstract Dextransucrase from __Leuconostoc mesenteroides__ was produced in a semicontinuous culture with slow addition of a concentrated sucrose solution. The resulting high activity of the fermentation broth allowed a oneβstep purification method, by gel permeation chromatography (GPC) in 96.4%
High activities of the enzyme dextransucrase were repeatedly produced using slowly agitated non-aerated fed-batch fermentations of Leuconostoc mesenteroides B-512(F). Activities in excess of 24.0 U cm-3 were obtained consistently in a 16 dm3 laboratory fermenter using a 6 dm3 initial work volume. Ye