On the interpretation of proton NMR spectra from brain tumours in vivo and in vitro

✍ Scribed by H. Bruhn; T. Michaelis; K. D. Merboldt; W. Hänicke; M. L. Gyngell; C. Hamburger; J. Frahm

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 748 KB
• Volume: 5
• Category: Article
• ISSN: 0952-3480
• DOI: 10.1002/nbm.1940050510

✦ Synopsis

Localized proton NMR spectroscopy in uiuo allows focal studies of cerebral metabolites in both man and laboratory animals from image-defined regions as small as 1 mL or 64 p ~, respectively. Althougk brain tumours lead to remarkable spectral alterations relative to normal brain, a number of problems may compromise the interpretation of the results. Potential complications arise from the chosen experimental conditions (method, TE, size and location of volume of interest), from regional metabolic heterogeneity in and around tumours, from differences between human tumours and animal models, and from discrepancies between in uiuo and in uitro findings. Strategies and pitfalls are illustrated with use of selected examples from primary brain tumours, a rat tumour model and perchloric acid extracts of resected specimens.