On the heterogeneity of the slow reassociating (“unique”) DNA

✍ Scribed by Ivan G. Ivanov; George G. Markov

• Book ID: 104678927
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 491 KB
• Volume: 20
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00241389

✦ Synopsis

The slow reassociating fraction of mouse DNA ("unique DNA"), when allowed to reassociate in 0.14 M sodoum phosphate buffer at 50 degrees C showed a biphasic melting curve with a transition at 78--80 degrees C. On the basis of this feature, the slow reassociating DNA was separated preparatively into two fractions: "unique DNA" I and II. Their duplexes showed differences with respect to thermal stability, S1 nuclease resistance and rate of reassociation. About one third of the sequences in each fraction were fraction-specific. The conclusion was drawn that for "unique DNA" I these should be the low repetitive or single copy related sequences (multigene families) and for "unique DNA" II--the unrelated single copy sequences or recent families of low repetitive not yet diverged sequences.