On the genuineness of citrus essential oils. Part LIII. Determination of the composition of the oxygen heterocyclic fraction of lemon essential oils (Citrus limon (L.) Burm. F.) by normal-phase high performance liquid chromatography
✍ Scribed by Paola Dugo; Luigi Mondello; Eugenia Cogliandro; Antonella Cavazza; Giovanni Dugo
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 140 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0882-5734
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✦ Synopsis
The oxygen heterocyclic fraction of cold-pressed lemon essential oils has been studied by normalphase HPLC. The components of the fraction have been isolated by column chromatography, TLC and semipreparative HPLC with recycle. The identi®cation of the isolated components has been carried out by 1 H-NMR and mass spectrometry. Three coumarins (5-geranyloxy-7-methoxycoumarin, citropten, 5-isopentenyloxy-7-methoxycoumarin) and ten psoralens (bergamottin, 8-geranyloxypsoralen, oxypeucedanin, byakangelicol, oxypeucedanin hydrate, byakangelicin, imperatorin, phellopterin, isoimperatorin, 5-isopent-2 H -enyloxy-8-(2 H ,3 H -epoxyisopentyloxy)-psoralen) have been isolated and identi®ed. The main components were bergamottin (160±291 mg/100 g of oil) and 5-geranyloxy-7-methoxycoumarin (180±250 mg/100 g of oil). Moreover, herniarin, a coumarin characteristic of lime essential oil, has been detected. Herniarin had been reported previously in essential oils obtained from Citrus limon L. var. Eureka.