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On the art of identifying effective and specific siRNAs

✍ Scribed by Pei, Yi; Tuschl, Thomas

Book ID
109937998
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
215 KB
Volume
3
Category
Article
ISSN
1552-4450

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## Abstract Small interfering RNA (siRNA) molecules achieve sequence‐specific gene silencing through the RNA interference (RNAi) mechanism. Here, live‐cell and live‐animal bioluminescent imaging (BLI) is used to directly compare luciferase knockdown by unmodified and nuclease‐stabilized siRNAs in r