On-site gluing system makes assembly easier

A Proposal for Using Modified Site-speci

A Proposal for Using Modified Site-specific Recombination Systems for Making Insertions into a Chosen Chromosomal Sitein vivoBased on the Analysis of I Phage Integration

✍ Paweł Sachadyn; Józef Kur 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 415 KB

A proposal is presented here to use modified mobile genetic elements for making chromosomal insertions in vivo, into any desired chromosomal site. The idea lies in replacing the sequences which direct specificity of integration of those elements with other DNA sequences, homologous to the desired ta